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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
22/03/2016 |
Data da última atualização: |
19/06/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PÉREZ-MONTAÑO, F.; CERRO, P. del; JIMÉNEZ-GUERRERO, I.; LÓPEZ-BAENA, F. J.; CUBO, M. T.; HUNGRIA, M.; MEGÍAS, M.; OLLERO, F. J. |
Afiliação: |
Francisco Péres-Montaño, Universidade de Sevilla; Pablo del Cerro, Universidade de Sevilia; Irene Jiménez-Guerreiro, Universidade de Sevilla; Francisco Javier López-Baena, Universidade de Sevilla; maria Teresa Cubo, Universidade de Sevilla; MARIANGELA HUNGRIA DA CUNHA, CNPSO; Manuel Megías, Universidade de Sevilla; Francisco Javier Ollero, Uiversidade de Sevilla. |
Título: |
RNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
BMC Genomics, v. 17, n. 1, p. 198, Mar. 2016. |
ISSN: |
1471-2164 |
DOI: |
10.1186/s12864-016-2543-3 |
Idioma: |
Inglês |
Conteúdo: |
Rhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the expression of nodulation genes in the absence of flavonoids. Thus, in the presence of both nodulation inducer molecules, apigenin and salt, R. tropici CIAT 899 up-regulated the same set of symbiotic genes. It could be possible that the increases in the transcription levels of several genes related to nodulation under saline conditions could represent a strategy to establish symbiosis under abiotic stressing conditions. MenosRhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the e... Mostrar Tudo |
Palavras-Chave: |
Lipochitooligosaccharides; Nod factors; Nodutlation; RNA-seq. |
Thesagro: |
Nodulação; Rhizobium. |
Thesaurus Nal: |
Apigenin; Rhizobium tropici; Salt stress. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142584/1/RNA-seq-analysis-of-the-rhizobium...pdf
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Marc: |
LEADER 02939naa a2200337 a 4500 001 2041600 005 2017-06-19 008 2016 bl uuuu u00u1 u #d 022 $a1471-2164 024 7 $a10.1186/s12864-016-2543-3$2DOI 100 1 $aPÉREZ-MONTAÑO, F. 245 $aRNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt.$h[electronic resource] 260 $c2016 520 $aRhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the expression of nodulation genes in the absence of flavonoids. Thus, in the presence of both nodulation inducer molecules, apigenin and salt, R. tropici CIAT 899 up-regulated the same set of symbiotic genes. It could be possible that the increases in the transcription levels of several genes related to nodulation under saline conditions could represent a strategy to establish symbiosis under abiotic stressing conditions. 650 $aApigenin 650 $aRhizobium tropici 650 $aSalt stress 650 $aNodulação 650 $aRhizobium 653 $aLipochitooligosaccharides 653 $aNod factors 653 $aNodutlation 653 $aRNA-seq 700 1 $aCERRO, P. del 700 1 $aJIMÉNEZ-GUERRERO, I. 700 1 $aLÓPEZ-BAENA, F. J. 700 1 $aCUBO, M. T. 700 1 $aHUNGRIA, M. 700 1 $aMEGÍAS, M. 700 1 $aOLLERO, F. J. 773 $tBMC Genomics$gv. 17, n. 1, p. 198, Mar. 2016.
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Embrapa Soja (CNPSO) |
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Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
25/10/2007 |
Data da última atualização: |
06/03/2008 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Circulação/Nível: |
-- - -- |
Autoria: |
ANGELINI, G. A. R.; PEREIRA, M. G.; SAGGIN JÚNIOR, O. J.; TORRES, J. L. R.; LOSS, A. |
Afiliação: |
Guilherme Augusto Robles Angelini, UFRRJ; Marcos Gervásio Pereira, UFRRJ; Orivaldo José Saggin Júnior, Embrapa Agrobiologia; J. L. R. Torres, CEFET-Uberaba; Arcângelo. Loss, UFRRJ. |
Título: |
Colonização micorrízica e densidade de esporos de fungos micorrízicos arbusculares em solo de cerrado sob plantio direto com diferentes coberturas vegetais. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 31., 2007, Gramado. Conquistas e desafios da ciência do solo brasileira. Porto Alegre: Universidade Federal do Rio Grande do Sul, 2007. |
Páginas: |
4 p. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Notas: |
Parceria: UFRRJ, CEFET-Uberaba |
Thesagro: |
Micorriza; Planta de Cobertura; Plantio Direto. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00853naa a2200217 a 4500 001 1629245 005 2008-03-06 008 2007 bl --- 0-- u #d 100 1 $aANGELINI, G. A. R. 245 $aColonização micorrízica e densidade de esporos de fungos micorrízicos arbusculares em solo de cerrado sob plantio direto com diferentes coberturas vegetais. 260 $c2007 300 $a4 p.$c1 CD-ROM. 500 $aParceria: UFRRJ, CEFET-Uberaba 650 $aMicorriza 650 $aPlanta de Cobertura 650 $aPlantio Direto 700 1 $aPEREIRA, M. G. 700 1 $aSAGGIN JÚNIOR, O. J. 700 1 $aTORRES, J. L. R. 700 1 $aLOSS, A. 773 $tIn: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 31., 2007, Gramado. Conquistas e desafios da ciência do solo brasileira. Porto Alegre: Universidade Federal do Rio Grande do Sul, 2007.
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